منابع مشابه
'Morphs' (MRFs): metal-reversible folding domains for differential IgG binding.
BACKGROUND Selective recognition and binding of IgG molecules is the basis for a host of immunological and affinity purification techniques. Capture of an IgG in these procedures relies chiefly on its interaction with one of a variety of reengineered bacterial receptors which bind to the Fc region of IgG molecules with very high affinity. While this interaction is extremely efficient in trappin...
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Dehydrins are hydrophilic proteins that are responsive to osmotic stress, such as drought, cold, and salinity in plants. Although they have been hypothesized to stabilize macromolecules in stressed cells, their functions are not fully understood. Citrus dehydrin, which accumulates mainly in response to cold stress, enhances cold tolerance in transgenic tobacco by reducing lipid peroxidation. It...
متن کاملCo-operative binding of histones to DNA.
The interaction of certain components of chromatin was studied in a model system. It was found that when calf thymus histones were mixed with sonicated phage DNA and the macromolecules made to interact by lowering the ionic strength, a mixture of complexes was formed, each separable by velocity sedimentation and characterized by unique thermal stability properties and histone contents. The bind...
متن کاملMultiple metal binding domains enhance the Zn(II) selectivity of the divalent metal ion transporter AztA.
Transition metal-transporting P1B-type CPx ATPases play crucial roles in mediating metal homeostasis and resistance in all cells. The degree to which N-terminal metal binding domains (MBDs) confer metal specificity to the transporter is unclear. We show that the two MBDs of the Zn/Cd/Pb effluxing pump Anabaena AztA are functionally nonequivalent, but only with respect to zinc resistance. Inacti...
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Ni(2+)-binding staphylococci were generated through surface display of combinatorially engineered variants of a fungal cellulose-binding domain (CBD) from Trichoderma reesei cellulase Cel7A. Novel CBD variants were generated by combinatorial protein engineering through the randomization of 11 amino acid positions, and eight potentially Ni(2+)-binding CBDs were selected by phage display technolo...
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ژورنال
عنوان ژورنال: Science
سال: 1986
ISSN: 0036-8075,1095-9203
DOI: 10.1126/science.3787266